Biomass Yield and Total Lipid Yield of Chlorella pyrenoidosa XQ-20044 Cultured at Different Dilution Rates Biodiesel is an environmentally friendly renewable energy source, but due to the severe shortage of raw materials, it restricts its industrial development. The growth and reproduction of microalgae is fast, and the oil yield is high. Non-agricultural land can be used for industrial production. It has many advantages as a raw material for biodiesel. Both domestic and foreign cultured oil microalgae adopt the batch culture mode. The cell growth and oil accumulation are completed in two steps. When the oil accumulates, the cell growth is severely inhibited, which inevitably reduces the oil yield and becomes the development of microalgae biodiesel. One of the technical difficulties. Breaking this technical bottleneck and realizing the simultaneous growth and oil production of microalgae is of great significance in improving the oil production efficiency of microalgae and promoting the industrialization of microalgae biodiesel. Wen Xiaobin, a PhD student in the Plant Biotechnology Division of the Wuhan Botanical Garden of the Chinese Academy of Sciences under the guidance of researcher Li Guangguang, conducted a study on the efficient oil production mode of microalgae, and used continuous culture to control the specific nitrate input rate. The simultaneous growth and oil production of Chlorella were successfully achieved, and a one-step production mode of oil production was established. Firstly, Chlorella pyrenoidosa XQ-20044, an oil-producing microalgae with potential for application, was batched and fed to nitrogen sources in batches and batches. It was found that the algae can accumulate oil under moderate nitrogen stress conditions. , and not at the expense of growth rate and biomass dry weight. Further, continuous culture was used to culture Chlorella pyrenoidosa XQ-20044 in a columnar photoreactor at a constant temperature, light intensity, pH, and CO2 supply. When the dilution rate was 0.48-1.44d-1, the chlorella reached a stable growth state (Steady-state) with a growth rate of 0.48-1.44d-1, and the content of intracellular triacylglycerols increased significantly, ie, it grew rapidly. Chlorella cells can also accumulate lipids, and the key factor regulating this simultaneous growth and accumulation of lipids is the ratio of nitrogen source (nitrogen nitrate) input. Under the experimental conditions, the specific input rate of sodium nitrate for the synchronous growth and oil production of C.pyrenoidosa XQ-20044 is 0.78-4.56 mmol g-1DWd-1, and the oil yield is up to 144.93 mgL-1d-1, under the same conditions. Compared with batch culture, the oil yield increased by 48% and there was no significant difference in cell fatty acid composition. The results of this study demonstrated the feasibility and great potential of simultaneous growth and oil production of microalgae, and opened up a new way to increase the oil production efficiency of microalgae. The study was supported by the National Natural Science Foundation of China and the national "863" project. Research papers are published in Bioresource Technology (doi:10.1016/j.biortech.2014.03.077). Lab Heat Circulation Bath means heat circulator for lab jacketed Chemical Glass Reactor heating . You can put water or oil into bath of heat circulation bath and it will circulate hot water or oil into jacket of reactors to make mixture reaction . Temperature Cycling,Thermal Cycle System,Lab Heat Circulation Bath,Laboratory Temperature Cycling Zhongyi Kori(Zhengzhou)Equipment Co.,Ltd , https://www.korireactor.com
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Lab heat circulation bath with different size at 10l 20l 30l 50l 100l 150l and 200l