The high performance liquid chromatography column rechromatography system plays a role in separating the detection substances, just like the heart of the chromatographic system, and is also a lossy product. Because the column is in the process of separating the sample, the adsorption of the different components in the column is different, so that the components are separated, and the column contains the salt, lipid and hydrophobic protein in the sample when the sample is adsorbed. And some other biological substances may interact with the column. Some substances with smaller retention values, such as salts, are easier to be washed out of the column, while some retentively strong mobile phase solution components are not enough to elute these substances. After several times of loading, the substances adsorbed on the surface of the column usually accumulate in the column head. When these adsorbed sample components accumulate to a certain extent enough to form a new stationary phase and separate the mechanism, the sample retention time fluctuates and a tailing phenomenon occurs. The direct performance of the chromatographic column of the high-performance liquid chromatograph is the deterioration of the chromatographic peak shape, broadening, asymmetry, tailing and column efficiency, which affects the accuracy of the chromatographic data. The main reason for the inability of the column to continue to be used is that the strong retained material in the sample gradually accumulates in the column during long-term use, and the serious situation directly causes the column to be scrapped. To extend the life of the column, the commonly used method is column regeneration. The regeneration of high-performance liquid chromatography column is to re-treat the column which is seriously polluted during normal use and can not effectively remove the contaminated impurities by the conventional cleaning method, thereby removing pollutants and obviously improving the performance indexes of the column. Return it to a series of cleaning processes that continue to be in use. The column regeneration method is mainly selected according to the stationary phase. The methods selected according to different stationary phases are as follows: Reversed-phase column: The mobile phase is respectively washed with a solvent such as methanol:water=90:10, pure methanol, dichloromethane, etc., and washed successively, and each mobile phase flows through the column by not less than 20 times the column volume. Then rinse in the reverse order. Normal phase column: use a solvent such as n-hexane, isopropanol, dichloromethane or methanol as the mobile phase, and rinse sequentially. Each mobile phase flows through the column at a column volume of not less than 20 times (the viscosity of isopropanol is large). , can reduce the flow rate and avoid excessive pressure). Note that the order in which the solvents are used should not be reversed. After rinsing with methanol, rinse to n-hexane in the reverse order. All mobile phases must be strictly dehydrated. Ion exchange column: Long-term use and injection in a buffer solution will result in a decrease in column ion exchange capacity. The cation column can be regenerated by rinsing with a dilute acid buffer solution, whereas the anion column can be regenerated by rinsing with a dilute alkali buffer solution. The steps for column regeneration are as follows: 1. First reverse the column; 2. Rinse the column at a flow rate of approximately 40% of the optimum flow rate for approximately 45-60 minutes, using the solvent in the order of water-methanol-isopropanol-dichloromethane-isopropanol-methanol-water; 3. The column is turned back to the normal direction and equilibrated with the eluent used in the analysis. Not all retired columns can be successfully regenerated, and the column is regenerated. Lu Chuang analysis believes that the quality of the column can be significantly improved to a certain extent, so that it can be restored to a usable state, even after a very successful regeneration. It is also impossible for the column to return to the level of the new column. The column of the liquid chromatograph will be used for a certain period of time and the column will not be regenerated. 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